Effect of S-region mutations on HBsAg in HBsAg-negative HBV-infected patients.

ABSTRACT:

Background

Occult HBV infection (OBI) is a special form of hepatitis B virus (HBV) infection that may cause Liver cirrhosis and hepatocellular carcinoma, causing significant harm to patients. Given the insidious nature of OBI, it is usually not easy to be detected. Most of the samples currently studied are concentrated on blood donors, however, patients in this special state have not been fully studied. This project aimed to study the effect of HBV S region mutations on HBsAg in patients with clinical OBI.

Methods

Collect 107 HBsAg-/HBV DNA + blood samples from Beijing Youan Hospital, Capital Medical University from August 2022 to April 2023. Next, the successfully extracted and amplified HBV DNA S regions were sequenced. Construct mutant plasmids to verify the cell function

SUBMITTER: Liu H 

PROVIDER: S-EPMC11040738 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature