The characteristics of microbiome in the upper respiratory tract of COVID-19 patients.

ABSTRACT:

Background

Co-infection with other pathogens in coronavirus disease 2019 (COVID-19) patients exacerbates disease severity and impacts patient prognosis. Clarifying the exact pathogens co-infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is premise of the precise treatment for COVID-19 patients.

Methods

Sputum samples were collected from 17 patients in the COVID-19 positive group and 18 patients in the COVID-19 negative group. DNA extraction was performed to obtain the total DNA. Sequencing analysis using 16S and ITS rRNA gene was carried out to analyze the composition of bacterial and fungal communities. Meanwhile, all the samples were inoculated for culture.

Results

We did not observe significant differences in bacterial composition between t

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11040800 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature