RT-QuIC detection of chronic wasting disease prion in platelet samples of white-tailed deer.

ABSTRACT:

Background

Chronic wasting disease (CWD) is a prion disease of captive and free-ranging cervids. Currently, a definitive diagnosis of CWD relies on immunohistochemistry detection of PrP^Sc in the obex and retropharyngeal lymph node (RPLN) of the affected cervids. For high-throughput screening of CWD in wild cervids, RPLN samples are tested by ELISA followed by IHC confirmation of positive results. Recently, real-time quacking-induced conversion (RT-QuIC) has been used to detect CWD positivity in various types of samples. To develop a blood RT-QuIC assay suitable for CWD diagnosis, this study evaluated the assay sensitivity and specificity with and without ASR1-based preanalytical enrichment and NaI as the main ionic component in assay buffer.

Results

A total of 23

SUBMITTER: Kobashigawa E 

PROVIDER: S-EPMC11041042 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature