Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy.

ABSTRACT: Conventional histology, as well as immunohistochemistry or immunofluorescence, enables the study of morphological and phenotypical changes during tissue inflammation with single-cell accuracy. However, although highly specific, such techniques require multiple time-consuming steps to apply exogenous labels, which might result in morphological deviations from native tissue structures. Unlike these techniques, mid-infrared (mid-IR) microspectroscopy is a label-free optical imaging method that retrieves endogenous biomolecular contrast without altering the native composition of the samples. Nevertheless, due to the strong optical absorption of water in biological tissues, conventional mid-IR microspectroscopy has been limited to dried thin (5-10 µm) tissue preparations and, thus, it also requ

SUBMITTER: Ko V 

PROVIDER: S-EPMC11041735 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature