Mix-method toolbox for monitoring greenhouse gas production and microbiome responses to soil amendments.

ABSTRACT: In this study, we adopt an interdisciplinary approach, integrating agronomic field experiments with soil chemistry, molecular biology techniques, and statistics to investigate the impact of organic residue amendments, such as vinasse (a by-product of sugarcane ethanol production), on soil microbiome and greenhouse gas (GHG) production. The research investigates the effects of distinct disturbances, including organic residue application alone or combined with inorganic N fertilizer on the environment. The methods assess soil microbiome dynamics (composition and function), GHG emissions, and plant productivity. Detailed steps for field experimental setup, soil sampling, soil chemical analyses, determination of bacterial and fungal community diversity, quantification of genes related to nitri

SUBMITTER: Lourenco KS 

PROVIDER: S-EPMC11041840 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature