Multidimensional analysis of cortical interneuron synaptic features reveals underlying synaptic heterogeneity.

ABSTRACT: Cortical interneurons represent a diverse set of neuronal subtypes characterized in part by their striking degree of synaptic specificity. However, little is known about the extent of synaptic diversity because of the lack of unbiased methods to extract synaptic features among interneuron subtypes. Here, we develop an approach to aggregate image features from fluorescent confocal images of interneuron synapses and their post-synaptic targets, in order to characterize the heterogeneity of synapses at fine scale. We started by training a model that recognizes pre- and post-synaptic compartments and then determines the target of each genetically-identified interneuron synapse in vitro and in vivo. Our model extracts hundreds of spatial and intensity features from each analyzed synapse, constr

SUBMITTER: Dummer PD 

PROVIDER: S-EPMC11042224 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature