Cross-reactivity of r<i>Pvs</i>48/45, a recombinant <i>Plasmodium vivax</i> protein, with sera from <i>Plasmodium falciparum</i> endemic areas of Africa.

ABSTRACT:

Background

Ps48/45, a Plasmodium gametocyte surface protein, is a promising candidate for malaria transmission-blocking (TB) vaccine. Due to its relevance for a multispecies vaccine, we explored the cross-reactivity and TB activity of a recombinant P. vivax Ps48/45 protein (rPvs48/45) with sera from P. falciparum-exposed African donors.

Methods

rPvs48/45 was produced in Chinese hamster ovary cell lines and tested by ELISA for its cross-reactivity with sera from Burkina Faso, Tanzania, Mali, and Nigeria - In addition, BALB/c mice were immunized with the rPvs48/45 protein formulated in Montanide ISA-51 and inoculated with a crude extract of P. falciparum NF-54 gametocytes to evaluate the parasite-boosting effect on rPv

SUBMITTER: Balam S 

PROVIDER: S-EPMC11042229 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature