PAbFold: Linear Antibody Epitope Prediction using AlphaFold2.

ABSTRACT: Defining the binding epitopes of antibodies is essential for understanding how they bind to their antigens and perform their molecular functions. However, while determining linear epitopes of monoclonal antibodies can be accomplished utilizing well-established empirical procedures, these approaches are generally labor- and time-intensive and costly. To take advantage of the recent advances in protein structure prediction algorithms available to the scientific community, we developed a calculation pipeline based on the localColabFold implementation of AlphaFold2 that can predict linear antibody epitopes by predicting the structure of the complex between antibody heavy and light chains and target peptide sequences derived from antigens. We found that this AlphaFold2 pipeline, which we call P

SUBMITTER: DeRoo J 

PROVIDER: S-EPMC11042291 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature