Organelle-targeted Laurdans measure heterogeneity in subcellular membranes and their responses to saturated lipid stress.

ABSTRACT: Cell organelles feature characteristic lipid compositions that lead to differences in membrane properties. In living cells, membrane ordering and fluidity are commonly measured using the solvatochromic dye Laurdan, whose fluorescence is sensitive to membrane packing. As a general lipophilic dye, Laurdan stains all hydrophobic environments in cells, so it is challenging to characterize membrane properties in specific organelles or assess their responses to pharmacological treatments in intact cells. Here, we describe the synthesis and application of Laurdan-derived probes that read out membrane packing of individual cellular organelles. The set of Organelle-targeted Laurdans (OTL) localizes to the ER, mitochondria, lysosomes and Golgi compartments with high specificity, while retaining the

SUBMITTER: Wong AM 

PROVIDER: S-EPMC11042318 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature