Fecal microbiota transplantation stimulates type 2 and tolerogenic immune responses in a mouse model.

ABSTRACT:

Objectives

Clostridioides difficile infection (CDI) is the leading hospital-acquired infection in North America. While previous work on fecal microbiota transplantation (FMT), a highly effective treatment for CDI, has focused on colonization resistance mounted against C. difficile by FMT-delivered commensals, the effects of FMT on host gene expression are relatively unexplored. This study aims to identify transcriptional changes associated with FMT, particularly changes associated with protective immune responses.

Methods

Gene expression was assessed on day 2 and day 7 after FMT in mice after antibiotic-induced dysbiosis. Flow cytometry was also performed on colon and mesenteric lymph nodes at day 7 to investigate changes in immune cell populations.

Results

FMT admini

SUBMITTER: Moreau GB 

PROVIDER: S-EPMC11042976 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature