Identification of New Markers of Angiogenic Sprouting Using Transcriptomics: New Role for RND3.

ABSTRACT:

Background

New blood vessel formation requires endothelial cells to transition from a quiescent to an invasive phenotype. Transcriptional changes are vital for this switch, but a comprehensive genome-wide approach focused exclusively on endothelial cell sprout initiation has not been reported.

Methods

Using a model of human endothelial cell sprout initiation, we developed a protocol to physically separate cells that initiate the process of new blood vessel formation (invading cells) from noninvading cells. We used this model to perform multiple transcriptomics analyses from independent donors to monitor endothelial gene expression changes.

Results

Single-cell population analyses, single-cell cluster analyses, and bulk RNA sequencing revealed common transcriptomic chan

SUBMITTER: Abbey CA 

PROVIDER: S-EPMC11043006 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature