Thyroid hormone induces ossification and terminal maturation in a preserved OA cartilage biomimetic model.

ABSTRACT:

Objective

To characterize aspects of triiodothyronine (T3) induced chondrocyte terminal maturation within the molecular osteoarthritis pathophysiology using the previously established T3 human ex vivo osteochondral explant model.

Designs

RNA-sequencing was performed on explant cartilage obtained from OA patients (n = 8), that was cultured ex vivo with or without T3 (10 ng/ml), and main findings were validated using RT-qPCR in an independent sample set (n = 22). Enrichment analysis was used for functional clustering and comparisons with available OA patient RNA-sequencing and GWAS datasets were used to establish relevance for OA pathophysiology by linking to OA patient genomic profiles.

Results

Besides the upregulation of known hypertrophic genes EPAS1 and ANKH, T3 tre

SUBMITTER: Korthagen NM 

PROVIDER: S-EPMC11044551 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature