NMR characterization of RNA binding property of the DEAD-box RNA helicase DDX3X and its implications for helicase activity.

ABSTRACT: The DEAD-box RNA helicase (DDX) plays a central role in many aspects of RNA metabolism by remodeling the defined structure of RNA molecules. While a number of structural studies have revealed the atomistic details of the interaction between DDX and RNA ligands, the molecular mechanism of how this molecule unwinds a structured RNA into an unstructured single-stranded RNA (ssRNA) has largely remained elusive. This is due to challenges in structurally characterizing the unwinding intermediate state and the lack of thermodynamic details underlying this process. In this study, we use solution nuclear magnetic resonance (NMR) spectroscopy to characterize the interaction of human DDX3X, a member of the DDX family, with various RNA ligands. Our results show that the inherent binding affinity of DD

SUBMITTER: Toyama Y 

PROVIDER: S-EPMC11045745 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature