Single-cell adhesive profiling in an optofluidic device elucidates CD8⁺ T lymphocyte phenotypes in inflamed vasculature-like microenvironments.

ABSTRACT: Tissue infiltration by circulating leukocytes occurs via adhesive interactions with the local vasculature, but how the adhesive quality of circulating cells guides the homing of specific phenotypes to different vascular microenvironments remains undefined. We developed an optofluidic system enabling fluorescent labeling of photoactivatable cells based on their adhesive rolling velocity in an inflamed vasculature-mimicking microfluidic device under physiological fluid flow. In so doing, single-cell level multidimensional profiling of cellular characteristics could be characterized and related to the associated adhesive phenotype. When applied to CD8⁺ T cells, ligand/receptor expression profiles and subtypes associated with adhesion were revealed, providing insight into inflamed t

SUBMITTER: Camargo CP 

PROVIDER: S-EPMC11046032 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature