A network pharmacology-based approach to explore the molecular mechanism of Aidi injection against prostate cancer.

ABSTRACT:

Objective

To explore the molecular mechanism of Aidi injection in the treatment of prostate cancer (PCa).

Materials and methods

CCK-8 and colony formation assays were used to detect the effects of Aidi on PC3 and DU145 cells; effects on the cell cycle and apoptosis of DU145 cells were detected by flow cytometry; effects on migration and invasion of PC3 and DU145 cells were detected by wound healing and transwell assay, respectively. The main active components of Aidi, their corresponding targets, and PCa associated pathways were predicted and analyzed by network pharmacology. Then predicted key targets and related signaling pathways were further verified by western blotting. The potential active components of Aidi were predicted by molecular docking technology.

Results

SUBMITTER: Guo S 

PROVIDER: S-EPMC11046112 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature