Liver specification of human iPSC-derived endothelial cells transplanted into mouse liver.

ABSTRACT:

Background & aims

Liver sinusoidal endothelial cells (LSECs) are important in liver development, regeneration, and pathophysiology, but the differentiation process underlying their tissue-specific phenotype is poorly understood and difficult to study because primary human cells are scarce. The aim of this study was to use human induced pluripotent stem cell (hiPSC)-derived LSEC-like cells to investigate the differentiation process of LSECs.

Methods

hiPSC-derived endothelial cells (iECs) were transplanted into the livers of Fah^-/-/Rag2^-/-/Il2rg^-/- mice and assessed over a 12-week period. Lineage tracing, immunofluorescence, flow cytometry, plasma human factor VIII measurement, and bulk and single cell

SUBMITTER: Yap KK 

PROVIDER: S-EPMC11046210 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature