SF3B3-regulated mTOR alternative splicing promotes colorectal cancer progression and metastasis.

ABSTRACT:

Background

Aberrant alternative splicing (AS) is a pervasive event during colorectal cancer (CRC) development. SF3B3 is a splicing factor component of U2 small nuclear ribonucleoproteins which are crucial for early stages of spliceosome assembly. The role of SF3B3 in CRC remains unknown.

Methods

SF3B3 expression in human CRCs was analyzed using publicly available CRC datasets, immunohistochemistry, qRT-PCR, and western blot. RNA-seq, RNA immunoprecipitation, and lipidomics were performed in SF3B3 knockdown or overexpressing CRC cell lines. CRC cell xenografts, patient-derived xenografts, patient-derived organoids, and orthotopic metastasis mouse models were utilized to determine the in vivo role of SF3B3 in CRC progression and metastasis.

Results

SF3B3 was upregulated

SUBMITTER: Xu T 

PROVIDER: S-EPMC11047005 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature