A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis.

ABSTRACT: Genetically encoded circuits have been successfully utilized to assess and characterize target variants with desirable traits from large mutant libraries. Adenosylcobalamin is an essential coenzyme that is required in many intracellular physiological reactions and is widely used in the pharmaceutical and food industries. High-throughput screening techniques capable of detecting adenosylcobalamin productivity and selecting superior adenosylcobalamin biosynthesis strains are critical for the creation of an effective microbial cell factory for the production of adenosylcobalamin at an industrial level. In this study, we developed an RNA-protein hybrid biosensor whose input part was an endogenous RNA riboswitch to specifically respond to adenosylcobalamin, the inverter part was an orthogonal t

SUBMITTER: Yang X 

PROVIDER: S-EPMC11047186 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature