Cytoplasmic domain of Sendai virus HN protein contains a specific sequence required for its incorporation into virions.

ABSTRACT: In the assembly of paramyxoviruses, interactions between viral proteins are presumed to be specific. The focus of this study is to elucidate the protein-protein interactions during the final stage of viral assembly that result in the incorporation of the viral envelope proteins into virions. To this end, we examined the specificity of HN incorporation into progeny virions by transiently transfecting HN cDNA genes into Sendai virus (SV)-infected cells. SV HN expressed from cDNA was efficiently incorporated into progeny Sendai virions, whereas Newcastle disease virus (NDV) HN was not. This observation supports the theory of a selective mechanism for HN incorporation. To identify the region on HN responsible for the selective incorporation, we constructed chimeric SV and NDV HN cDNAs and eval

SUBMITTER: Takimoto T 

PROVIDER: S-EPMC110485 | biostudies-literature | 1998 Dec

REPOSITORIES: biostudies-literature