Mucosal prime-boost immunization with live murine pneumonia virus-vectored SARS-CoV-2 vaccine is protective in macaques.

ABSTRACT: Immunization via the respiratory route is predicted to increase the effectiveness of a SARS-CoV-2 vaccine. Here, we evaluate the immunogenicity and protective efficacy of one or two doses of a live-attenuated murine pneumonia virus vector expressing SARS-CoV-2 prefusion-stabilized spike protein (MPV/S-2P), delivered intranasally/intratracheally to male rhesus macaques. A single dose of MPV/S-2P is highly immunogenic, and a second dose increases the magnitude and breadth of the mucosal and systemic anti-S antibody responses and increases levels of dimeric anti-S IgA in the airways. MPV/S-2P also induces S-specific CD4⁺ and CD8⁺ T-cells in the airways that differentiate into large populations of tissue-resident memory cells within a month after the boost. One dose induc

SUBMITTER: Kaiser JA 

PROVIDER: S-EPMC11053155 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature