Development of a TaqMan Real-Time PCR for Early and Accurate Detection of Anthracnose Pathogen <i>Colletotrichum siamense</i> in <i>Pachira glabra</i>.

ABSTRACT: Anthracnose, caused by Colletotrichum siamense, is a destructive disease of Pachira glabra in southern China. Early and proper monitoring and quantification of C. siamense is of importance for disease control. A calmodulin (CAL) gene-based TaqMan real-time PCR assay was developed for efficient detection and quantification of C. siamense, which reliably detected as low as 5 pg of genomic DNA and 12.8 fg (5800 copies) of target DNA. This method could specifically recognize all tested C. siamense isolates, while no amplification was observed in other closely related Colletotrichum species. The assay could still detect C. siamense in plant mixes, of which only 0.01% of the tissue was infected. A dynamic change in the amount of C. siame

SUBMITTER: Gu J 

PROVIDER: S-EPMC11054339 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature