VP4 Mutation Boosts Replication of Recombinant Human/Simian Rotavirus in Cell Culture.

ABSTRACT: Rotavirus A (RVA) is the leading cause of diarrhea requiring hospitalization in children and causes over 100,000 annual deaths in Sub-Saharan Africa. In order to generate next-generation vaccines against African RVA genotypes, a reverse genetics system based on a simian rotavirus strain was utilized here to exchange the antigenic capsid proteins VP4, VP7 and VP6 with those of African human rotavirus field strains. One VP4/VP7/VP6 (genotypes G9-P[6]-I2) triple-reassortant was successfully rescued, but it replicated poorly in the first cell culture passages. However, the viral titer was enhanced upon further passaging. Whole genome sequencing of the passaged virus revealed a single point mutation (A797G), resulting in an amino acid exchange (E263G) in VP4. After introducing this mutation int

SUBMITTER: Valusenko-Mehrkens R 

PROVIDER: S-EPMC11054354 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature