Understanding and fine tuning the propensity of ATP-driven liquid-liquid phase separation with oligolysine.

ABSTRACT: Liquid-liquid phase separation (LLPS) plays a pivotal role in the organization and functionality of living cells. It is imperative to understand the underlying driving forces behind LLPS and to control its occurrence. In this study, we employed coarse-grained (CG) simulations as a research tool to investigate systems comprising oligolysine and adenosine triphosphate (ATP) under conditions of various ionic concentrations and oligolysine lengths. Consistent with experimental observations, our CG simulations captured the formation of LLPS upon the addition of ATP and tendency of dissociating under high ionic concentration. The electrostatic interaction between oligolysine and ATP is of great importance in forming LLPS. An in-depth analysis on the structural properties of LLPS was conducted, w

SUBMITTER: Zhu Q 

PROVIDER: S-EPMC11056285 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature