Spatially resolved quantification of oxygen consumption rate in ex vivo lymph node slices.

ABSTRACT: Cellular metabolism has been closely linked to activation state in cells of the immune system, and the oxygen consumption rate (OCR) in particular serves as a valuable metric for assessing metabolic activity. Several oxygen sensing assays have been reported for cells in standard culture conditions. However, none have provided a spatially resolved, optical measurement of local oxygen consumption in intact tissue samples, making it challenging to understand regional dynamics of consumption. Therefore, here we established a system to monitor the rates of oxygen consumption in ex vivo tissue slices, using murine lymphoid tissue as a case study. By integrating an optical oxygen sensor into a sealed perfusion chamber and incorporating appropriate correction for photobleaching of the senso

SUBMITTER: Anbaei P 

PROVIDER: S-EPMC11056769 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature