Pla2g5 contributes to viral-like-induced lung inflammation through macrophage proliferation and LA/Ffar1 lung cell recruitment.

ABSTRACT: Macrophages expressing group V phospholipase A₂ (Pla2g5) release the free fatty acid (FFA) linoleic acid (LA), potentiating lung type 2 inflammation. Although Pla2g5 and LA increase in viral infections, their role remains obscure. We generated Pla2g5^flox/flox mice, deleted Pla2g5 by using the Cx3cr1^cre transgene, and activated bone marrow-derived macrophages (BM-Macs) with poly:IC, a synthetic double-stranded RNA that triggers a viral-like immune response, known Pla2g5-dependent stimuli (IL-4, LPS + IFNγ, IL-33 + IL-4 + GM-CSF) and poly:IC + LA followed by lipidomic and transcriptomic analysis. Poly:IC-activated Pla2g5^flox/flox;Cx3cr1^cre/+ BM-Macs had downregulation of major bioactive lipids and critical enzymes producing those bioacti

SUBMITTER: Koganesawa M 

PROVIDER: S-EPMC11057362 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature