The non-template functions of helper virus RNAs create optimal replication conditions to enhance the proliferation of satellite RNAs.

ABSTRACT: As a type of parasitic agent, satellite RNAs (satRNAs) rely on cognate helper viruses to achieve their replication and transmission. During the infection of satRNAs, helper virus RNAs serve as templates for synthesizing viral proteins, including the replication proteins essential for satRNA replication. However, the role of non-template functions of helper virus RNAs in satRNA replication remains unexploited. Here we employed the well-studied model that is composed of cucumber mosaic virus (CMV) and its associated satRNA. In the experiments employing the CMV trans-replication system, we observed an unexpected phenomenon the replication proteins of the mild strain LS-CMV exhibited defective in supporting satRNA replication, unlike those of the severe strain Fny-CMV. Independent of translati

SUBMITTER: Qiao Z 

PROVIDER: S-EPMC11057728 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature