Proteomic analysis reveals a potential role for extracellular vesicles within the erythroblastic island niche.

ABSTRACT: Introduction: Erythroblastic island (EBI) macrophages play an essential role in the production and maturation of the vast numbers of red blood cells (RBCs) that are produced throughout life. Their location within the bone marrow makes it difficult to study the cellular and molecular interactions associated with their action so we have used an in vitro model of the EBI niche using macrophages derived from human induced pluripotent stem cells (hiPSCs). We previously demonstrated that the activation of the transcription factor KLF1 enhanced the activity of hiPSC-derived EBI macrophages. Methods: To elucidate the mechanisms associated with EBI-like activity we carried out a quantitative proteomic analysis and assessed the role of extracellular vesicles using Nanosight Trac

SUBMITTER: Ventura T 

PROVIDER: S-EPMC11058792 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature