Delineating the heterogeneity of senescence-induced-functional alterations in hepatocytes.

ABSTRACT:

Background and aim

Cellular senescence of hepatocytes involves permanent cell cycle arrest, disrupted cellular bioenergetics, resistance to cell death, and the release of pro-inflammatory cytokines. This 'zombie-like' state perpetuates harmful effects on tissues and holds potential implications for liver disease progression. Remarkably, senescence exhibits heterogeneity, stemming from two crucial factors: the inducing stressor and the cell type. As such, our present study endeavors to characterize stressor-specific changes in senescence phenotype, its related molecular patterns, and cellular bioenergetics in primary mouse hepatocytes (PMH) and hepatocyte-derived liver organoids (HepOrgs).

Methods

PMH, isolated by collagenase-perfused mouse liver (C57B6/J; 18-23 weeks), were

SUBMITTER: Kumar P 

PROVIDER: S-EPMC11058795 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature