Quantification of Adaptive Immune Responses Against Protein-Binding Interfaces in the Streptococcal M1 Protein.

ABSTRACT: Bacterial or viral antigens can contain subdominant protein regions that elicit weak antibody responses upon vaccination or infection although there is accumulating evidence that antibody responses against subdominant regions can enhance the protective immune response. One proposed mechanism for subdominant protein regions is the binding of host proteins that prevent antibody production against epitopes hidden within the protein binding interfaces. Here, we used affinity purification combined with quantitative mass spectrometry (AP-MS) to examine the level of competition between antigen-specific antibodies and host-pathogen protein interaction networks using the M1 protein from Streptococcus pyogenes as a model system. As most humans have circulating antibodies against the M1 protein, we f

SUBMITTER: Torres-Sangiao E 

PROVIDER: S-EPMC11059317 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature