A high efficiency precision genome editing method with CRISPR in iPSCs.

ABSTRACT: The use of genetic engineering to generate point mutations in induced pluripotent stem cells (iPSCs) is essential for studying a specific genetic effect in an isogenic background. We demonstrate that a combination of p53 inhibition and pro-survival small molecules achieves a homologous recombination rate higher than 90% using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) in human iPSCs. Our protocol reduces the effort and time required to create isogenic lines.

SUBMITTER: Singh A 

PROVIDER: S-EPMC11061145 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature