Directed Differentiation of Human Induced Pluripotent Stem Cells to Heart Valve Cells.

ABSTRACT:

Background

A main obstacle in current valvular heart disease research is the lack of high-quality homogeneous functional heart valve cells. Human induced pluripotent stem cells (hiPSCs)-derived heart valve cells may help with this dilemma. However, there are no well-established protocols to induce hiPSCs to differentiate into functional heart valve cells, and the networks that mediate the differentiation have not been fully elucidated.

Methods

To generate heart valve cells from hiPSCs, we sequentially activated the Wnt, BMP4, VEGF (vascular endothelial growth factor), and NFATc1 signaling pathways using CHIR-99021, BMP4, VEGF-165, and forskolin, respectively. The transcriptional and functional similarity of hiPSC-derived heart valve cells compared with primary heart valve ce

SUBMITTER: Cai Z 

PROVIDER: S-EPMC11062615 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature