DengueSeq: a pan-serotype whole genome amplicon sequencing protocol for dengue virus.

ABSTRACT:

Background

The increasing burden of dengue virus on public health due to more explosive and frequent outbreaks highlights the need for improved surveillance and control. Genomic surveillance of dengue virus not only provides important insights into the emergence and spread of genetically diverse serotypes and genotypes, but it is also critical to monitor the effectiveness of newly implemented control strategies. Here, we present DengueSeq, an amplicon sequencing protocol, which enables whole-genome sequencing of all four dengue virus serotypes.

Results

We developed primer schemes for the four dengue virus serotypes, which can be combined into a pan-serotype approach. We validated both approaches using genetically diverse virus stocks and clinical specimens that contained a r

SUBMITTER: Vogels CBF 

PROVIDER: S-EPMC11062901 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature