Novel tetracycline resistance gene tet(65) located on a multi-resistance Corynebacterium plasmid.

ABSTRACT:

Background

Corynebacterium (C.) sp. 22KM0430 related to C. oculi and isolated from a dog exhibited resistance to tetracycline, and its WGS analysis revealed a putative resistance gene on a 35 562-bp plasmid also harbouring the MLSB resistance gene erm(X).

Objectives

To characterize the novel tetracycline resistance gene tet(65) and demonstrate its functionality by expression in C. glutamicum and Escherichia coli and plasmid curing of the host strain.

Methods

tet(65) was cloned with and without its repressor tetR(65) and expressed in C. glutamicum DSM20300 and E. coli DH5α. Plasmid was cured by non-selective passages. Minimal inhibitory concentrations (MICs) of tetracyclines were determined according to CLSI guidelines. Association of tet(65) with efflux was shown by t

SUBMITTER: Kittl S 

PROVIDER: S-EPMC11062942 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature