Zebrafish cobll1a regulates lipid homeostasis via the RA signaling pathway.

ABSTRACT:

Background

The COBLL1 gene has been implicated in human central obesity, fasting insulin levels, type 2 diabetes, and blood lipid profiles. However, its molecular mechanisms remain largely unexplored.

Methods

In this study, we established cobll1a mutant lines using the CRISPR/Cas9-mediated gene knockout technique. To further dissect the molecular underpinnings of cobll1a during early development, transcriptome sequencing and bioinformatics analysis was employed.

Results

Our study showed that compared to the control, cobll1a ^-/- zebrafish embryos exhibited impaired development of digestive organs, including the liver, intestine, and pancreas, at 4 days post-fertilization (dpf). Transcriptome sequencing and bioinformatics analysis

SUBMITTER: Zeng T 

PROVIDER: S-EPMC11063382 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature