Classical and novel properties of Holliday junction resolvase SynRuvC from Synechocystis sp. PCC6803.

ABSTRACT: Cyanobacteria, which have a photoautotrophic lifestyle, are threatened by ultraviolet solar rays and the reactive oxygen species generated during photosynthesis. They can adapt to environmental conditions primarily because of their DNA damage response and repair mechanisms, notably an efficient homologous recombination repair system. However, research on double-strand break (DSB) repair pathways, including the Holliday junction (HJ) resolution process, in Synechocystis sp. PCC6803 is limited. Here, we report that SynRuvC from cyanobacteria Synechocystis sp. PCC6803 has classical HJ resolution activity. We investigated the structural specificity, sequence preference, and biochemical properties of SynRuvC. SynRuvC strongly preferred Mn²⁺ as a cofactor, and its cleava

SUBMITTER: Gu Y 

PROVIDER: S-EPMC11063404 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature