Rapid generation of human recombinant monoclonal antibodies from antibody-secreting cells using ferrofluid-based technology.

ABSTRACT: Monoclonal antibodies (mAbs) are one of the most important classes of biologics with high therapeutic and diagnostic value, but traditional methods for mAbs generation, such as hybridoma screening and phage display, have limitations, including low efficiency and loss of natural chain pairing. To overcome these challenges, novel single B cell antibody technologies have emerged, but they also have limitations such as in vitro differentiation of memory B cells and expensive cell sorters. In this study, we present a rapid and efficient workflow for obtaining human recombinant monoclonal antibodies directly from single antigen-specific antibody secreting cells (ASCs) in the peripheral blood of convalescent COVID-19 patients using ferrofluid technology. This process allows the identificat

SUBMITTER: Strazza V 

PROVIDER: S-EPMC11064063 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature