Transferable AmpCs in <i>Klebsiella pneumoniae</i>: interplay with peptidoglycan recycling, mechanisms of hyperproduction, and virulence implications.

ABSTRACT: Chromosomal and transferable AmpC β-lactamases represent top resistance mechanisms in different gram-negatives, but knowledge regarding the latter, mostly concerning regulation and virulence-related implications, is far from being complete. To fill this gap, we used Klebsiella pneumoniae (KP) and two different plasmid-encoded AmpCs [DHA-1 (AmpR regulator linked, inducible) and CMY-2 (constitutive)] as models to perform a study in which we show that blockade of peptidoglycan recycling through AmpG permease inactivation abolished DHA-1 inducibility but did not affect CMY-2 production and neither did it alter KP pathogenic behavior. Moreover, whereas regular production of both AmpC-type enzymes did not attenuate KP virulence, when blaDHA-1 was expressed in an ampG-defecti

SUBMITTER: Barcelo IM 

PROVIDER: S-EPMC11064642 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature