Powdery mildew effectors AVR_A1 and BEC1016 target the ER J-domain protein HvERdj3B required for immunity in barley.

ABSTRACT: The barley powdery mildew fungus, Blumeria hordei (Bh), secretes hundreds of candidate secreted effector proteins (CSEPs) to facilitate pathogen infection and colonization. One of these, CSEP0008, is directly recognized by the barley nucleotide-binding leucine-rich-repeat (NLR) receptor MLA1 and therefore is designated AVR_A1. Here, we show that AVR_A1 and the sequence-unrelated Bh effector BEC1016 (CSEP0491) suppress immunity in barley. We used yeast two-hybrid next-generation interaction screens (Y2H-NGIS), followed by binary Y2H and in planta protein-protein interactions studies, and identified a common barley target of AVR_A1 and BEC1016, the endoplasmic reticulum (ER)-localized J-domain protein HvERdj3B. Silencing of this ER quality control (ERQC) protein

SUBMITTER: Li Z 

PROVIDER: S-EPMC11064805 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature