Comparative transcriptome analysis of bone marrow resident versus culture-expanded mouse mesenchymal stem/stromal cells.

ABSTRACT:

Background aims

Mesenchymal stem/stromal cells (MSCs) are defined as culture-expanded populations, and although these cells recapitulate many properties of bone marrow (BM) resident skeletal stem/progenitor cells, few studies have directly compared these populations to evaluate how culture adaptation and expansion impact critical quality attributes.

Methods

We analyzed by RNA sequencing Lin^-SCA1⁺ MSCs enriched from BM by immunodepletion (ID) and after subsequent culture expansion (Ex) and Lin^-LEPR⁺ MSCs sorted (S) directly from BM. Pairwise comparisons were used to identify differentially expressed genes (DEGs) between populations, and gene set enrichment analysis was employed to identify biological pathways/processes unique to eac

SUBMITTER: Haga CL 

PROVIDER: S-EPMC11065607 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature