A new suite of reporter vectors and a novel landing site survey system to study cis-regulatory elements in diverse insect species.

ABSTRACT: Comparative analyses between traditional model organisms, such as the fruit fly Drosophila melanogaster, and more recent model organisms, such as the red flour beetle Tribolium castaneum, have provided a wealth of insight into conserved and diverged aspects of gene regulation. While the study of trans-regulatory components is relatively straightforward, the study of cis-regulatory elements (CREs, or enhancers) remains challenging outside of Drosophila. A central component of this challenge has been finding a core promoter suitable for enhancer-reporter assays in diverse insect species. Previously, we demonstrated that a Drosophila Synthetic Core Promoter (DSCP) functions in a cross-species manner in Drosophila and Tribolium. Given the over 300 million years of divergence between the Dipter

SUBMITTER: Deem KD 

PROVIDER: S-EPMC11066043 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature