NEK2 promotes the migration, invasion, proliferation of ESCC and mediates ESCC immunotherapy.

ABSTRACT:

Purpose

Esophageal squamous cell carcinoma (ESCC) is a disease with a high incidence rate and high mortality worldwide. The Never in Mitosis A (NIMA) family member NIMA-related kinase 2 (NEK2) plays an important role in mitosis. However, the role of NEK2 in the pathogenesis of ESCC remains unclear.

Patients and methods

The expression and function of NEK2 in TCGA and GEO data sets were analyzed by bioinformatics. We verified the expression of NEK2 in ESCC tissues and cell lines by Western blotting and immunohistochemical methods and further explored the relationship between tumor stage and NEK2 expression. The differences in NEK2 expression and survival in patients with EC were verified by bioinformatics analysis. ESCC cell lines with stable knockdown of NEK2 were established

SUBMITTER: Gu S 

PROVIDER: S-EPMC11066149 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature