Protocol to process crosslinking and immunoprecipitation data into annotated binding sites.

ABSTRACT: Here, we present a protocol for using Skipper, a pipeline designed to process crosslinking and immunoprecipitation (CLIP) data into annotated binding sites. We describe steps for partitioning annotated transcript regions and fitting data to a beta-binomial model to call windows of enriched binding. From raw CLIP data, we detail how users can map reproducible RNA-binding sites to call enriched windows and perform downstream analysis. This protocol supports optional customizations for different use cases. For complete details on the use and execution of this protocol, please refer to Boyle et al.¹.

SUBMITTER: Xu S 

PROVIDER: S-EPMC11066461 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature