Characterization of plant produced V_HH antibodies against cobra venom toxins for antivenom therapy.

ABSTRACT: Cobra (Naja kaouthia) venom contains many toxins including α-neurotoxin (αNTX) and phospholipase A2 (PLA2), which can cause neurodegeneration, respiratory failure, and even death. The traditional antivenom derived from animal serum faces many challenges and limitations. Heavy-chain-only antibodies (HCAb), fusing V_HH with human IgG Fc region, offer advantages in tissue penetration, antigen binding, and extended half-life. This research involved the construction and transient expression of two types of V_HH-F_C which are specific to α-Neurotoxin (V_HH-αNTX-F_C) and Phospholipase A2 (V_HH-PLA2-F_C) in Nicotiana benthamiana leaves. The recombinant HCAbs were incubated for up to six days to optimize expression

SUBMITTER: Vitayathikornnasak S 

PROVIDER: S-EPMC11066514 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature