DNMT3A-mediated DNA methylation and transcription inhibition of FZD5 suppresses lung carcinogenesis.

ABSTRACT:

Background

Based on the bioinformatics prediction, this study investigates the correlation between aberrant transcription factor Frizzled 5 (FZD5) expression and the establishment of non-small cell lung cancer (NSCLC).

Methods

A mouse model with regard to primary NSCLC was encouraged by intraperitoneal injection of urethane. Lentivirus-based FZD5 silencing was then administrated to examine its role in tumorigenesis in the mouse lung. Silencing of FZD5 was induced in two NSCLC cell lines to examine its function in the malignant behavior pertaining to cells in vitro. Quantitative methylation-specific PCR was employed to assess the DNA methylation level within the NSCLC cells. DNA methyltransferases (DNMTs) that administer FZD5 were assessed by chromatin immunoprecipitat

SUBMITTER: Shen J 

PROVIDER: S-EPMC11066612 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature