Chemical Proteomics Strategies for Analyzing Protein Lipidation Reveal the Bacterial <i>O</i>-Mycoloylome.

ABSTRACT: Protein lipidation dynamically controls protein localization and function within cellular membranes. A unique form of protein O-fatty acylation in Corynebacterium, termed protein O-mycoloylation, involves the attachment of mycolic acids─unusually large and hydrophobic fatty acids─to serine residues of proteins in these organisms' outer mycomembrane. However, as with other forms of protein lipidation, the scope and functional consequences of protein O-mycoloylation are challenging to investigate due to the inherent difficulties of enriching and analyzing lipidated peptides. To facilitate the analysis of protein lipidation and enable the comprehensive profiling and site mapping of protein O-mycoloylation, we developed a chemical proteomics strategy integrat

SUBMITTER: Banahene N 

PROVIDER: S-EPMC11066868 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature