STOPGAP: an open-source package for template matching, subtomogram alignment and classification.

ABSTRACT: Cryo-electron tomography (cryo-ET) enables molecular-resolution 3D imaging of complex biological specimens such as viral particles, cellular sections and, in some cases, whole cells. This enables the structural characterization of molecules in their near-native environments, without the need for purification or separation, thereby preserving biological information such as conformational states and spatial relationships between different molecular species. Subtomogram averaging is an image-processing workflow that allows users to leverage cryo-ET data to identify and localize target molecules, determine high-resolution structures of repeating molecular species and classify different conformational states. Here, STOPGAP, an open-source package for subtomogram averaging that is designed to pr

SUBMITTER: Wan W 

PROVIDER: S-EPMC11066880 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature