Structural and functional reorganization of inhibitory synapses by activity-dependent cleavage of neuroligin-2.

ABSTRACT: Recent evidence has demonstrated that the transsynaptic nanoscale organization of synaptic proteins plays a crucial role in regulating synaptic strength in excitatory synapses. However, the molecular mechanism underlying this transsynaptic nanostructure in inhibitory synapses still remains unclear and its impact on synapse function in physiological or pathological contexts has not been demonstrated. In this study, we utilized an engineered proteolysis technique to investigate the effects of acute cleavage of neuroligin-2 (NL2) on synaptic transmission. Our results show that the rapid cleavage of NL2 led to impaired synaptic transmission by reducing both neurotransmitter release probability and quantum size. These changes were attributed to the dispersion of RIM1/2 and GABA_A rece

SUBMITTER: Xu N 

PROVIDER: S-EPMC11067042 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature