Identification of the potassium-binding site in serotonin transporter.

ABSTRACT: Clearance of serotonin (5-hydroxytryptamine, 5-HT) from the synaptic cleft after neuronal signaling is mediated by serotonin transporter (SERT), which couples this process to the movement of a Na⁺ ion down its chemical gradient. After release of 5-HT and Na⁺ into the cytoplasm, the transporter faces a rate-limiting challenge of resetting its conformation to be primed again for 5-HT and Na⁺ binding. Early studies of vesicles containing native SERT revealed that K⁺ gradients can provide an additional driving force, via K⁺ antiport. Moreover, under appropriate conditions, a H⁺ ion can replace K⁺. Intracellular K⁺ accelerates the resetting step. Structural studies of SERT have identified two binding sites f

SUBMITTER: Hellsberg E 

PROVIDER: S-EPMC11067047 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature