Metagenomic nanopore sequencing for exploring the nature of antimicrobial metabolites of Bacillus haynesii.

ABSTRACT: Multidrug-resistant (MDR) pathogens are a rising global health worry that imposes an urgent need for the discovery of novel antibiotics particularly those of natural origin. In this context, we aimed to use the metagenomic nanopore sequence analysis of soil microbiota coupled with the conventional phenotypic screening and genomic analysis for identifying the antimicrobial metabolites produced by promising soil isolate(s). In this study, whole metagenome analysis of the soil sample(s) was performed using MinION™ (Oxford Nanopore Technologies). Aligning and analysis of sequences for probable secondary metabolite gene clusters were extracted and analyzed using the antiSMASH version 2 and DeepBGC. Results of the metagenomic analysis showed the most abundant taxa were Bifidobacterium, Burkholde

SUBMITTER: Eltokhy MA 

PROVIDER: S-EPMC11069495 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature