Basophils are important for development of allergic skin inflammation.

ABSTRACT:

Background

Atopic dermatitis skin lesions exhibit increased infiltration by basophils. Basophils produce IL-4, which plays an important role in the pathogenesis of atopic dermatitis.

Objective

We sought to determine the role of basophils in a mouse model of antigen-driven allergic skin inflammation.

Methods

Wild-type mice, mice with selective and inducible depletion of basophils, and mice expressing Il4-driven enhanced green fluorescent protein were subjected to epicutaneous sensitization with ovalbumin or saline. Sensitized skin was examined by histology for epidermal thickening. Cells were analyzed for surface markers and intracellular expression of enhanced green fluorescent protein by flow cytometry. Gene expression was evaluated by real-time reverse transcription

SUBMITTER: Leyva-Castillo JM 

PROVIDER: S-EPMC11070311 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature